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Mouse Interleukin 18 Binding Protein (IL-18BP) ELISA Kit

SKU:
MODL00687
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z0M9
Sensitivity:
45pg/mL
Range:
125-8000pg/mL
ELISA Type:
Sandwich
Synonyms:
IL18BPa,MC51L-53L-54L Homolog Gene Product,Tadekinig-alfa
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Interleukin 18 Binding Protein (IL-18BP) ELISA Kit

The Mouse Interleukin-18 Binding Protein (IL18BP) ELISA Kit is a specialized assay designed for the precise measurement of IL18BP levels in mouse serum, plasma, and cell culture supernatants. This kit delivers exceptional sensitivity and specificity, ensuring consistent and dependable results for various research purposes.IL18BP is a key protein that binds to interleukin-18, inhibiting its activity and modulating immune responses. This interaction has implications in inflammatory processes, autoimmune diseases, and infectious conditions, making IL18BP a valuable biomarker for studying immune regulation and developing targeted therapies.

With its advanced technology and reliable performance, the Mouse IL18BP ELISA Kit is an essential tool for researchers investigating the roles of IL18BP in immune function and disease pathogenesis. Get accurate and reproducible results with this innovative assay kit from AssayGenie.

Product Name:Mouse Interleukin 18 Binding Protein (IL-18BP) ELISA Kit
Product Code:MODL00687
Size:96 Assays
Target:IL18BP
Synonyms:IL18BPa, MC51L-53L-54L Homolog Gene Product, Tadekinig-alfa
Detection Method:Sandwich
Range:125-8,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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