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Mouse Interleukin 10 (IL-10) ELISA Kit

SKU:
MODL00672
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P18893
Sensitivity:
34.5pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
CSIF,IL10A,TGIF,Cytokine Synthesis Inhibitory Factor
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

system_update_altDatasheet

Mouse Interleukin 10 (IL-10) ELISA Kit

The Mouse Interleukin-10 (IL-10) ELISA Kit is a powerful tool for the precise quantification of interleukin-10 levels in mouse serum, plasma, and tissue lysate samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.Interleukin-10 is a key regulatory cytokine that plays a crucial role in immune response modulation, inflammation regulation, and autoimmune diseases. Its dysregulation is associated with various pathologies, including inflammatory disorders, infectious diseases, and cancer.

Therefore, measuring IL-10 levels can provide valuable insights into immune function and disease progression.By utilizing the Mouse Interleukin-10 (IL-10) ELISA Kit, researchers can gain a deeper understanding of the complex roles of IL-10 in immune regulation and disease pathogenesis, paving the way for potential therapeutic interventions and personalized medicine approaches.

Product Name:Mouse Interleukin 10 (IL-10) ELISA Kit
Product Code:MODL00672
Size:96 Assays
Target:IL10
Synonyms:CSIF, IL10A, TGIF, Cytokine Synthesis Inhibitory Factor
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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