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Mouse Interferon Alpha (IFNa) ELISA Kit

SKU:
MODL00658
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01572
Sensitivity:
6.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
IFNA1,IFL,LeIF D,IFN,IFN-Alpha,IFNA13,IFN-A,IFNAP22,IFN-Alpha 1b,Interferon Alpha 1b,Interferon, Leukocytic,IFN, Leukocyte,Interferon alpha-D
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Mouse Interferon Alpha (IFNa) ELISA Kit

The Mouse Interferon Alpha (IFN-α) ELISA Kit is a reliable and accurate tool for the quantification of IFN-α levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for precise measurement of IFN-α concentrations. Interferon Alpha is a key cytokine involved in the immune response, playing a crucial role in antiviral defense, immune modulation, and anti-tumor activity. Measuring IFN-α levels can provide valuable insights into immune system function, viral infections, autoimmune diseases, and cancer research.

With its user-friendly protocol and robust performance, the Mouse Interferon Alpha (IFN-α) ELISA Kit is an essential tool for researchers studying immunology, infectious diseases, and therapeutic development. Trust in this kit to deliver consistent and reliable results for your research needs.

Product Name:Mouse Interferon Alpha (IFNa) ELISA Kit
Product Code:MODL00658
Size:96 Assays
Target:IFNa
Synonyms:IFNA1, IFL, LeIF D, IFN, IFN-Alpha, IFNA13, IFN-A, IFNAP22, IFN-Alpha 1b, Interferon Alpha 1b, Interferon, Leukocytic, IFN, Leukocyte, Interferon alpha-D
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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