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Mouse Integrin Beta 3 (ITGb3) ELISA Kit

SKU:
MODL00747
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O54890
Sensitivity:
13.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD61,ITG-B3,GP3A,GP3-A,GpIIIa,Platelet Membrane Glycoprotein IIIa
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Integrin Beta 3 (ITGb3) ELISA Kit

The Mouse Integrin Beta 3 (ITGB3) ELISA Kit is specially designed to detect and quantify levels of ITGB3 in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.ITGB3 is a key integrin subunit involved in cell adhesion and signaling processes, playing a crucial role in cell migration, proliferation, and differentiation.

Dysregulation of ITGB3 expression has been implicated in various diseases such as cancer, thrombosis, and inflammatory disorders, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.With its reliable performance and user-friendly protocols, the Mouse ITGB3 ELISA Kit is an indispensable tool for researchers exploring the role of integrins in biological processes and disease pathology.

Product Name:Mouse Integrin Beta 3 (ITGb3) ELISA Kit
Product Code:MODL00747
Size:96 Assays
Target:ITGb3
Synonyms:CD61, ITG-B3, GP3A, GP3-A, GpIIIa, Platelet Membrane Glycoprotein IIIa
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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