null

Mouse Insulin Like Protein 3 (INSL3) ELISA Kit

SKU:
MODL00727
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O09107
Sensitivity:
6.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
RLNL,RLF,Relaxin-Like Factor,Leydig Cell,Leydig insulin-like peptide
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Insulin Like Protein 3 (INSL3) ELISA Kit

The Mouse Insulin-Like Protein 3 (INSL3) ELISA Kit is specifically designed for the accurate measurement of INSL3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results, making it an excellent choice for various research applications.INSL3 is a vital hormone involved in reproductive function, playing a crucial role in testicular descent during fetal development and regulating Leydig cell function in adult males.

Dysregulation of INSL3 has been associated with infertility and other reproductive disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse INSL3 ELISA Kit is a powerful tool for researchers studying reproductive biology and looking to understand the role of INSL3 in various physiological processes.

Product Name:Mouse Insulin Like Protein 3 (INSL3) ELISA Kit
Product Code:MODL00727
Size:96 Assays
Target:INSL3
Synonyms:RLNL, RLF, Relaxin-Like Factor,Leydig Cell, Leydig insulin-like peptide
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products