The Mouse IL-6R (Interleukin-6 receptor) ELISA Kit is a reliable and accurate tool for detecting IL-6 receptor levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.IL-6 receptor is an important component in the IL-6 signaling pathway, playing a key role in immune response, inflammation, and other physiological processes. Dysregulation of IL-6 receptor is associated with various diseases, including inflammatory disorders, autoimmune diseases, and cancer, making it a valuable biomarker for studying these conditions and potential therapeutic development.
Overall, the Mouse IL-6R ELISA Kit provides researchers with a powerful tool to investigate the role of IL-6 receptor in health and disease, ultimately advancing our understanding of various pathologies and potential treatment strategies.
Product Name:
Mouse IL-6R ELISA Kit
SKU:
MOES01731
Target:
Mouse IL-6R
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-6R. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-6R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-6R, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-6R. You can calculate the concentration of Mouse IL-6R in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
89-105
98-115
Average (%)
103
96
105
1:4
Range (%)
89-101
86-101
85-100
Average (%)
95
93
91
1:8
Range (%)
88-104
85-97
84-96
Average (%)
96
91
89
1:16
Range (%)
90-105
80-95
83-96
Average (%)
97
87
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
86-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.78
249.53
941.68
97.45
233.71
904.11
Standard deviation
5.6
10.85
50.1
5.63
10.68
36.07
C V (%)
5.29
4.35
5.32
5.78
4.57
3.99
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-6R concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-6R in samples. No significant cross-reactivity or interference between Mouse IL-6R and analogues was observed.
