The Mouse IL1R1 CLIA Kit is specifically designed for the precise measurement of IL1R1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for a variety of research purposes.IL1R1, also known as Interleukin-1 Receptor 1, plays a critical role in the immune response by mediating the effects of interleukin-1.
It is involved in inflammation, immune responses, and various diseases such as autoimmune disorders and inflammatory conditions. Understanding the levels of IL1R1 can provide valuable insights into these processes, making this kit a valuable tool for studying and potentially treating these conditions.
Product Name:
Mouse IL-1R1 CLIA Kit
SKU:
MOES00015
Target:
Mouse IL-1R1
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1R1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1R1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1R1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-1R1. You can calculate the concentration of Mouse IL-1R1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
91-105
90-103
Average (%)
93
98
95
1:4
Range (%)
88-103
99-114
102-119
Average (%)
94
107
110
1:8
Range (%)
97-112
102-120
94-108
Average (%)
105
109
101
1:16
Range (%)
88-103
92-105
99-112
Average (%)
96
100
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
94-111
102
Cell culture media (n=5)
95-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.72
227.92
685.18
82.8
241.04
650.2
Standard deviation
9.85
21.88
73.38
9.58
20.3
65.15
C V (%)
10.74
9.6
10.71
11.57
8.42
10.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-1R1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-1R1 in samples. No significant cross-reactivity or interference between Mouse IL-1R1 and analogues was observed.
