Mouse IL 28A Recombinant Protein (RPPB0650)
- SKU:
- RPPB0650
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- Q4VK74
- Research Area:
- Cytokines
Description
| Product Name: | Mouse IL 28A Recombinant Protein |
| Product Code: | RPPB0650 |
| Size: | 20µg |
| Species: | Mouse |
| Target: | IL 28A |
| Synonyms: | IFN-lambda-2, Interleukin-28A, IL-28A, Ifnl2, Il28a, EG330496. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The Mouse IL-28A is lyophilized from a 0.2�m filtered concentrated solution in PBS, pH 7.4 and 5% trehalose. |
| Solubility: | It is recommended to reconstitute the lyophilized IL-28A in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IFN-Lambda 2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-Lambda 2 Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV |
| Biological Activity: | The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis(EMC) virus is less than 3 ng/ml, corresponding to a specific activity of > 3.3 � 105 IU/mg. |
IL-28A is distantly related to the IL-10 family. Expression of IL-28A is induced by viral infection which interacts with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha. IL-28A exhibits common features with type I IFNs such as antiviral activity, antiproliferative activity and in vivo antitumour activity.IL-28A acts similarly to IFNs, but is less effective generally and has activity in a more limited range of cell lines. IFN-ambda 1, IFN-lambda 2 and IFN-lambda 3 are closely positioned genes on human chromosome 19.IL-28A induces ELR(-) CXC chemokine mRNA in human peripheral blood mononuclear cells, in an IFN-gamma-independent manner. IL-28A is able to generate tolerogenic DCs, an activity that could thwart IFN-beta functions. IL-28A produced in response to viral infection, activates both monocytes and macrophages producing a restricted panel of cytokines and therefore is an important factor in activating innate immune responses at the site of viral infection.
Recombinant Mouse Interleukin-28A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids and having a total molecular mass of 19.7kDa.The IL-28A is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL28A: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.Protein type: Cytokine; Secreted, signal peptide; SecretedCellular Component: extracellular space; extracellular regionMolecular Function: cytokine activityBiological Process: positive regulation of immune response; mucosal immune response; JAK-STAT cascade; defense response to virus |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q4VK74 |
| NCBI GenInfo Identifier: | 408360147 |
| NCBI Gene ID: | 330496 |
| NCBI Accession: | Q4VK74.2 |
| UniProt Secondary Accession: | Q4VK74,E9Q2S2 |
| UniProt Related Accession: | Q4VK74 |
| Molecular Weight: | 21,740 Da |
| NCBI Full Name: | Interferon lambda-2 |
| NCBI Synonym Full Names: | interferon lambda 2 |
| NCBI Official Symbol: | Ifnl2�� |
| NCBI Official Synonym Symbols: | Il28a; IL-28A; EG330496�� |
| NCBI Protein Information: | interferon lambda-2; IL-28A; IFN-lambda2; IFN-lambda-2; interleukin 28A; interleukin-28A; interferon-lambda2 |
| UniProt Protein Name: | Interferon lambda-2 |
| UniProt Synonym Protein Names: | Interleukin-28A |
| Protein Family: | |
| UniProt Gene Name: | Ifnl2�� |
| UniProt Entry Name: | IFNL2_MOUSE |
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