Mouse IL 2 Recombinant Protein (RPPB0482)
- SKU:
- RPPB0482
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- P04351
- Research Area:
- Cytokines
Description
| Product Name: | Mouse IL 2 Recombinant Protein |
| Product Code: | RPPB0482 |
| Size: | 20µg |
| Species: | Mouse |
| Target: | IL 2 |
| Synonyms: | Interleukin-2, T-cell growth factor (TCGF),� Lymphokine, IL-2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution (1mg/ml) in 20mM PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized Mouse IL-2 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ |
| Biological Activity: | The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less than 0.2ng/ml corresponding to a Specific Activity of 5x106IU/mg. |
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Interleukin-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 148 amino acids and having a molecular mass of 17.2kDa. The IL-2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Oncoprotein; Cytokine Cellular Component: cytosol; extracellular region; extracellular space Molecular Function:carbohydrate binding; cytokine activity; glycosphingolipid binding; growth factor activity; interleukin-2 receptor binding; kappa-type opioid receptor binding Biological Process: adaptive immune response; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; immune response; immune system process; negative regulation of B cell apoptosis; negative regulation of heart contraction; negative regulation of inflammatory response; negative regulation of lymphocyte proliferation; negative regulation of protein amino acid phosphorylation; positive regulation of activated T cell proliferation; positive regulation of B cell proliferation; positive regulation of immunoglobulin secretion; positive regulation of interferon-gamma production; positive regulation of interleukin-17 production; positive regulation of isotype switching to IgG isotypes; positive regulation of protein amino acid phosphorylation; positive regulation of regulatory T cell differentiation; positive regulation of T cell differentiation; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat5 protein; protein kinase C activation; regulation of T cell homeostatic proliferation; response to ethanol |
| UniProt Code: | P04351 |
| NCBI GenInfo Identifier: | 124326 |
| NCBI Gene ID: | 16183 |
| NCBI Accession: | P04351.1 |
| UniProt Secondary Accession: | P04351,P97945, Q791T3, |
| UniProt Related Accession: | P04351 |
| Molecular Weight: | 19,400 Da |
| NCBI Full Name: | Interleukin-2 |
| NCBI Synonym Full Names: | interleukin 2 |
| NCBI Official Symbol: | Il2�� |
| NCBI Official Synonym Symbols: | Il-2�� |
| NCBI Protein Information: | interleukin-2 |
| UniProt Protein Name: | Interleukin-2 |
| UniProt Synonym Protein Names: | T-cell growth factor; TCGF |
| Protein Family: | Interleukin |
| UniProt Gene Name: | Il2�� |
| UniProt Entry Name: | IL2_MOUSE |
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