The Mouse Immunoglobulin M (IgM) ELISA Kit is engineered for the precise quantitative assessment of IgM levels in diverse biological samples. Immunoglobulin M, an essential component of the immune system, plays a fundamental role in the primary immune response to infections and antigens. As the first antibody class produced during an immune response, IgM is vital for agglutination and neutralization of pathogens.
This ELISA kit offers exceptional sensitivity and specificity, enabling accurate detection of IgM levels and enhancing the comprehension of its immune functions. With stringent quality control measures in place, the kit ensures dependable and reproducible outcomes, making it an ideal choice for research applications aiming to unravel the intricacies of immune responses orchestrated by IgM antibodies.
Product Name:
Mouse IgM (Immunoglobulin M) ELISA Kit
SKU:
AEES00231
Target:
Mouse IgM (Immunoglobulin M)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgM. You can calculate the concentration of Mouse IgM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-106
98-108
95-107
Average (%)
103
101
103
1:4
Range (%)
99-108
84-96
97-104
Average (%)
103
91
101
1:8
Range (%)
82-95
94-107
97-109
Average (%)
90
99
103
1:16
Range (%)
99-110
99-110
93-104
Average (%)
104
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-105
100
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
98-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
8.11
21.1
81.64
9.51
22.96
88.75
Standard deviation
0.45
1.04
3.83
0.5
1.14
4.14
C V (%)
5.55
4.93
4.69
5.26
4.97
4.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IgM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgM in samples. No significant cross-reactivity or interference between Mouse IgM and analogues was observed.
