The Mouse IgG3 (Immunoglobulin G3) ELISA Kit is specifically designed for the accurate quantification of IgG3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring dependable and consistent results, perfect for various research purposes.IgG3 is an essential subclass of immunoglobulins that plays a crucial role in the immune response by recognizing and binding to specific antigens, thereby mediating immune effector functions.
Elevated levels of IgG3 have been linked to certain autoimmune diseases, infections, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Mouse IgG3 ELISA Kit provides researchers with a reliable tool for investigating the role of IgG3 in immune responses and disease pathogenesis, ultimately advancing our understanding of these processes and improving diagnostics and treatment strategies.
Product Name:
Mouse IgG3 (Immunoglobulin G3) ELISA Kit
SKU:
MOES01682
Target:
Mouse IgG3 (Immunoglobulin G3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgG3. You can calculate the concentration of Mouse IgG3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-99
92-105
97-110
Average (%)
94
98
103
1:4
Range (%)
96-108
81-92
91-104
Average (%)
103
86
98
1:8
Range (%)
92-109
86-96
96-113
Average (%)
100
91
103
1:16
Range (%)
98-116
84-99
95-110
Average (%)
106
91
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
87-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.1
62.1
199.9
19.0
58.7
209.4
Standard deviation
1
2.5
8.4
1.1
2.8
10.3
C V (%)
4.98
4.03
4.2
5.79
4.77
4.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IgG3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgG3 in samples. No significant cross-reactivity or interference between Mouse IgG3 and analogues was observed.
