Mouse IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) ELISA Kit
The Mouse IGFBP-4 (Insulin-like Growth Factor Binding Protein 4) ELISA Kit is a valuable tool for accurately measuring levels of IGFBP-4 in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and reproducible results, making it an excellent choice for a variety of research applications.IGFBP-4 is a key protein involved in the regulation of insulin-like growth factors and plays a crucial role in controlling cell growth and development.
Its dysregulation has been linked to various diseases, including cancer and metabolic disorders, making it a vital biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse IGFBP-4 ELISA Kit offers researchers a reliable and efficient method for investigating the role of IGFBP-4 in biological processes and disease pathways, providing valuable insights for further research and drug development efforts.
Product Name:
Mouse IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) ELISA Kit
SKU:
MOES01203
Target:
Mouse IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGFBP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGFBP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IGFBP-4. You can calculate the concentration of Mouse IGFBP-4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
97-114
88-98
Average (%)
100
105
93
1:4
Range (%)
88-103
80-90
85-96
Average (%)
95
85
91
1:8
Range (%)
91-106
81-92
88-99
Average (%)
97
87
93
1:16
Range (%)
87-100
84-96
88-99
Average (%)
93
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.17
5.41
18.39
2.2
5.22
17.59
Standard deviation
0.15
0.24
0.95
0.11
0.28
0.93
C V (%)
6.91
4.44
5.17
5.0
5.36
5.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IGFBP-4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGFBP-4 in samples. No significant cross-reactivity or interference between Mouse IGFBP-4 and analogues was observed.
