Mouse IGFBP-3 (Insulin-like Growth Factor Binding Protein 3) ELISA Kit
The Mouse IGFBP-3 (Insulin-like Growth Factor Binding Protein 3) ELISA Kit is an advanced assay meticulously developed to quantitatively detect and analyze IGFBP-3 in various biological samples.
IGFBP-3, a crucial member of the insulin-like growth factor binding protein family, plays a significant role in modulating the actions of insulin-like growth factors (IGFs) and is involved in regulating cellular processes such as growth, proliferation, and apoptosis. Accurate measurement of IGFBP-3 levels is vital for gaining insights into its functions in regulating IGF signaling pathways, as well as its potential implications in growth, development, and various physiological processes. By quantifying IGFBP-3 expression, researchers can decipher its roles in cellular growth control, senescence, and the modulation of IGF activities. The Mouse IGFBP-3 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise, reproducible, and dependable results. Manufactified under stringent quality control standards, this kit is designed to deliver robust performance and ease of use, making it an ideal choice for research studies investigating the complex interplay between IGFBP-3, IGFs, and cellular processes.
Product Name:
Mouse IGFBP-3 (Insulin-like Growth Factor Binding Protein 3) ELISA Kit
SKU:
AEES00264
Target:
Mouse IGFBP-3 (Insulin-like Growth Factor Binding Protein 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGFBP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IGFBP-3. You can calculate the concentration of Mouse IGFBP-3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
94-107
96-113
Average (%)
98
99
104
1:4
Range (%)
93-110
89-101
95-110
Average (%)
101
96
101
1:8
Range (%)
94-110
90-105
97-113
Average (%)
101
96
105
1:16
Range (%)
90-104
93-105
96-106
Average (%)
95
99
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
102
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
95-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
229.27
547.07
1736.43
211.01
585.52
1576.02
Standard deviation
14.42
30.14
74.32
10.89
23.71
67.14
C V (%)
6.29
5.51
4.28
5.16
4.05
4.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IGFBP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGFBP-3 in samples. No significant cross-reactivity or interference between Mouse IGFBP-3 and analogues was observed.
