Mouse IGF-2 (Insulin Like Growth Factor 2) ELISA Kit
The Mouse IGF-2 (Insulin Like Growth Factor 2) ELISA Kit is a specialized assay designed for the precise and quantitative detection of IGF-2 levels in various biological samples. IGF-2 is a key growth factor that plays a significant role in cell proliferation, differentiation, and tissue growth. It is a potent stimulator of cell growth and plays essential roles in development, metabolism, and tissue repair processes. Accurate measurement of IGF-2 is crucial for understanding its involvement in growth regulation, developmental processes, and pathological conditions where its dysregulation may be implicated.
The IGF-2 ELISA kit provides researchers with a reliable tool to quantitatively analyze IGF-2 levels in biological samples, enabling detailed investigations into the mechanisms by which IGF-2 exerts its effects on cellular processes. Our Mouse IGF-2 ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research requirements. Manufactured under stringent quality control standards, this kit delivers robust performance and ease of use, making it an ideal choice for investigating the functional roles of IGF-2 in various biological contexts.
Product Name:
Mouse IGF-2 (Insulin Like Growth Factor 2) ELISA Kit
SKU:
AEES00266
Target:
Mouse IGF-2 (Insulin Like Growth Factor 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 pg/mL
Detection range:
0.78-50 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGF-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGF-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGF-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IGF-2. You can calculate the concentration of Mouse IGF-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
98-111
88-102
Average (%)
96
105
94
1:4
Range (%)
90-106
100-115
87-101
Average (%)
97
105
93
1:8
Range (%)
89-99
94-109
89-105
Average (%)
94
100
96
1:16
Range (%)
87-101
93-109
85-99
Average (%)
92
101
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
95
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
97-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.63
6.88
23.44
2.55
6.7
24.0
Standard deviation
0.18
0.32
1.09
0.14
0.32
0.78
C V (%)
6.93
4.58
4.66
5.68
4.8
3.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IGF-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGF-2 in samples. No significant cross-reactivity or interference between Mouse IGF-2 and analogues was observed.
