The Mouse IFNα (Interferon Alpha) ELISA Kit is a cutting-edge assay meticulously crafted for the precise and quantitative detection of Interferon Alpha levels in a diverse range of biological samples.
Interferon Alpha is a vital cytokine that plays a pivotal role in the body's immune response by combating viral infections and regulating various immune responses. This essential protein is a key player in the antiviral defense mechanism, influencing immune surveillance, immune cell activation, and immune regulation. Accurate measurement of Interferon Alpha levels is crucial for unraveling its multifaceted functions in immune signaling, antiviral immunity, and inflammatory processes. The Mouse IFNα ELISA Kit enables researchers to explore the intricate role of Interferon Alpha in immune modulation, viral infection, and autoimmune disorders, shedding light on the underlying mechanisms of immune responses and potential therapeutic strategies. With exceptional sensitivity and specificity, the Mouse IFNα ELISA Kit by Assay Genie ensures dependable and reproducible results, empowering researchers to delve into the complexities of immune regulation and antiviral defense mechanisms. Manufactured under stringent quality control standards, this kit guarantees robust performance and user-friendly protocols, making it an ideal tool for investigating the dynamic interplay of Interferon Alpha in immune function and disease pathology. Choose Assay Genie's IFNα ELISA Kit to advance your research and gain profound insights into the immune-modulatory functions of Interferon Alpha.
Product Name:
Mouse IFNα (Interferon Alpha) ELISA Kit
SKU:
AEES00247
Target:
Mouse IFNα (Interferon Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFNα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFNα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFNα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFNα. You can calculate the concentration of Mouse IFNα in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
94-109
89-103
Average (%)
95
101
94
1:4
Range (%)
89-102
96-111
84-95
Average (%)
95
104
90
1:8
Range (%)
91-106
96-111
85-97
Average (%)
98
102
92
1:16
Range (%)
89-104
97-111
90-105
Average (%)
96
104
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.31
114.27
450.06
52.89
117.3
463.36
Standard deviation
3.21
5.47
19.76
2.86
6.93
18.26
C V (%)
6.5
4.79
4.39
5.41
5.91
3.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IFNα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IFNα in samples. No significant cross-reactivity or interference between Mouse IFNα and analogues was observed.
