The Mouse IFNα (Interferon Alpha) CLIA Kit is a state-of-the-art assay designed to quantify Interferon Alpha levels in mouse samples with exceptional precision and accuracy. Interferon Alpha, a key type I interferon, plays a critical role in orchestrating the immune response against viral infections, tumors, and various immune-related pathways. Its potent antiviral properties and immunomodulatory functions are integral to regulating immune responses, highlighting its importance in immune surveillance, viral defense, and immune system equilibrium. Tailored for in-depth immune system investigations, the IFNα CLIA Kit provides researchers and clinicians with a powerful tool to accurately measure IFNα levels.
By enabling the quantitative analysis of Interferon Alpha, this kit supports detailed exploration of viral infections, autoimmune conditions, and the effectiveness of immunotherapy strategies. Characterized by remarkable sensitivity and specificity, the Mouse IFNα CLIA Kit delivers precise and reproducible results, ensuring dependable performance and accurate quantification of IFNα levels in diverse mouse samples. Manufactured under stringent quality standards, this kit offers robust and user-friendly operation, making it an excellent choice for cutting-edge research, clinical diagnostics, and immunological studies.
Product Name:
Mouse IFNα (Interferon Alpha) CLIA Kit
SKU:
AEES00730
Target:
Mouse IFNα (Interferon Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFNα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFNα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFNα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IFNα. You can calculate the concentration of Mouse IFNα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-111
90-103
90-105
Average (%)
105
95
98
1:4
Range (%)
92-103
99-111
93-104
Average (%)
96
104
101
1:8
Range (%)
91-100
97-107
85-97
Average (%)
97
101
92
1:16
Range (%)
101-109
85-96
96-105
Average (%)
105
92
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
91
EDTA plasma (n=5)
96-104
100
Cell culture media (n=5)
92-98
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.02
106.47
491.93
47.91
119.21
437.55
Standard deviation
2.82
5.21
22.68
2.69
5.41
18.73
C V (%)
5.87
4.89
4.61
5.61
4.54
4.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IFNα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IFNα in samples. No significant cross-reactivity or interference between Mouse IFNα and analogues was observed.
