The Mouse Intercellular Adhesion Molecule 1 (ICAM-1/CD54) ELISA Kit is meticulously crafted for the precise quantification of ICAM-1 levels in a variety of biological samples. ICAM-1, a crucial cell surface glycoprotein, is pivotal in mediating cell adhesion and plays a significant role in immune responses, inflammation, and cellular interactions. Its expression is tightly regulated and can serve as a valuable marker for immune system activation and inflammatory processes.
This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate detection of ICAM-1 levels and providing insights into its involvement in immune responses and inflammatory conditions. With stringent quality control measures, the kit guarantees reliable and reproducible outcomes, making it an excellent tool for research endeavors focused on understanding the functions and implications of ICAM-1 in biological systems.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ICAM-1/CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ICAM-1/CD54. You can calculate the concentration of Mouse ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
90-102
85-96
Average (%)
102
96
91
1:4
Range (%)
92-108
89-101
83-95
Average (%)
100
94
89
1:8
Range (%)
94-110
89-105
85-96
Average (%)
101
97
90
1:16
Range (%)
98-112
91-105
80-95
Average (%)
104
98
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
96
EDTA plasma (n=5)
88-103
95
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
7.65
21.34
216.38
8.13
19.27
226.04
Standard deviation
0.49
0.88
7.34
0.42
1.0
8.36
C V (%)
6.39
4.11
3.39
5.12
5.17
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ICAM-1/CD54 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Mouse ICAM-1/CD54 and analogues was observed.
