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Mouse Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) ELISA Kit

SKU:
MODL00628
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P00493
Sensitivity:
0.043ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
HGPRT,HGPRTase,HPRT,Lesch-Nyhan Syndrome,Hypoxanthine-guanine phosphoribosyltransferase
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) ELISA Kit

The Mouse HPRT1 (Hypoxanthine Phosphoribosyltransferase 1) ELISA Kit is specifically designed for the accurate measurement of HPRT1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.HPRT1 is a key enzyme involved in the salvage pathway of purine biosynthesis, playing a crucial role in DNA synthesis and repair.

Dysregulation of HPRT1 has been linked to various genetic disorders and diseases, making it a valuable biomarker for studying these conditions and potential therapeutic approaches.Overall, the Mouse HPRT1 ELISA Kit is an essential tool for researchers interested in studying purine metabolism, genetic disorders, and related diseases in mouse models.

Product Name:Mouse Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) ELISA Kit
Product Code:MODL00628
Size:96 Assays
Target:HPRT1
Synonyms:HGPRT, HGPRTase, HPRT, Lesch-Nyhan Syndrome, Hypoxanthine-guanine phosphoribosyltransferase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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