The Mouse HPA (Heparanase) CLIA Kit is a powerful tool for researchers looking to accurately measure levels of heparanase in mouse samples. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.Heparanase is an important enzyme that plays a key role in the breakdown of heparan sulfate, a crucial component of the extracellular matrix. Dysregulation of heparanase has been linked to various diseases, including cancer, inflammation, and diabetes, making it a valuable biomarker for studying these conditions and developing potential treatments.
With the Mouse HPA (Heparanase) CLIA Kit, researchers can gain valuable insights into the role of heparanase in disease pathology and explore new therapeutic strategies. Order your kit today and take your research to the next level.
Product Name:
Mouse HPA (Heparanase) CLIA Kit
SKU:
MOES00355
Target:
Mouse HPA (Heparanase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HPA. You can calculate the concentration of Mouse HPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
88-104
88-100
Average (%)
104
95
95
1:4
Range (%)
91-105
94-106
86-99
Average (%)
98
99
92
1:8
Range (%)
92-107
101-115
98-113
Average (%)
100
108
105
1:16
Range (%)
94-111
94-110
88-99
Average (%)
101
100
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.09
158.32
349.83
52.96
153.99
352.06
Standard deviation
6.17
13.66
23.09
6.42
17.49
23.41
C V (%)
11.84
8.63
6.6
12.12
11.36
6.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HPA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HPA in samples. No significant cross-reactivity or interference between Mouse HPA and analogues was observed.
