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Mouse HLA-B Associated Transcript 3 (BAT3) ELISA Kit

SKU:
MODL00145
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q3UF95
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
BAG6,Protein G3,Protein Scythe,BCL2-associated athanogene 6,BAG family molecular chaperone regulator 6,Large proline-rich protein BAG6
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse HLA-B Associated Transcript 3 (BAT3) ELISA Kit

The Mouse HLA-B Associated Transcript 3 (BAT3) ELISA Kit offered by Assay Genie is specifically designed for the accurate measurement of BAT3 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.BAT3 is a key protein involved in immune response regulation and cellular stress response. It plays a crucial role in the development and function of the immune system, making it an important biomarker for studying immune-related conditions and potential therapeutic interventions.

With the Mouse BAT3 ELISA Kit from Assay Genie, researchers can confidently investigate the role of BAT3 in immune modulation and disease progression, ultimately advancing our understanding of various health conditions and guiding the development of targeted treatments.

Product Name:Mouse HLA-B Associated Transcript 3 (BAT3) ELISA Kit
Product Code:MODL00145
Size:96 Assays
Target:BAT3
Synonyms:BAG6, Protein G3, Protein Scythe, BCL2-associated athanogene 6, BAG family molecular chaperone regulator 6, Large proline-rich protein BAG6
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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