Mouse Histidine Triad Nucleotide Binding Protein 2 (HINT2) ELISA Kit
The Mouse Histidine Triad Nucleotide Binding Protein 2 (HINT2) ELISA Kit is specifically designed for the precise measurement of HINT2 levels in mouse serum, plasma, and cell culture supernatants. With its excellent sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research applications. HINT2 is a critical protein involved in cellular processes, including apoptosis, cellular metabolism, and mitochondrial function. Dysregulation of HINT2 has been linked to various diseases such as cancer, metabolic disorders, and neurodegenerative conditions, highlighting its importance as a biomarker for investigating these pathologies and developing potential therapeutic interventions.
With its user-friendly protocol and reliable performance, the Mouse HINT2 ELISA Kit offers researchers a powerful tool for studying the role of HINT2 in disease pathogenesis and identifying novel therapeutic targets. Unlock new insights into cellular biology and disease mechanisms with this innovative ELISA kit.
Product Name:
Mouse Histidine Triad Nucleotide Binding Protein 2 (HINT2) ELISA Kit
Product Code:
MODL00622
Size:
96 Assays
Target:
HINT2
Synonyms:
HIT-17kDa, PKCI-1-related HIT protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
