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Mouse Hexosaminidase B Beta (HEXb) ELISA Kit

SKU:
MODL00617
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P20060
Sensitivity:
0.118ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
Hex-B,ENC-1AS,HCC-7,Beta-N-acetylhexosaminidase subunit beta,Cervical cancer proto-oncogene 7 protein,N-acetyl-beta-glucosaminidase subunit beta
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Hexosaminidase B Beta (HEXb) ELISA Kit

The Mouse Hexosaminidase B (Beta-HexB) ELISA Kit is specifically designed for the accurate measurement of Hexosaminidase B levels in mouse serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring precise and reliable results suitable for a variety of research applications.Hexosaminidase B, also known as Beta-HexB, is a key enzyme involved in glycosphingolipid degradation and lysosomal storage diseases. Mutations in the HEXB gene encoding Hexosaminidase B can result in disorders such as Tay-Sachs disease and Sandhoff disease, making this enzyme a crucial target for research in these areas.

By accurately quantifying Hexosaminidase B levels, researchers can gain valuable insights into lysosomal storage disorders, monitor disease progression, and potentially develop novel therapeutic strategies. This Mouse Hexosaminidase B ELISA Kit provides a reliable tool for studying these conditions and advancing our understanding of their underlying mechanisms.

Product Name:Mouse Hexosaminidase B Beta (HEXb) ELISA Kit
Product Code:MODL00617
Size:96 Assays
Target:HEXb
Synonyms:Hex-B, ENC-1AS, HCC-7, Beta-N-acetylhexosaminidase subunit beta, Cervical cancer proto-oncogene 7 protein, N-acetyl-beta-glucosaminidase subunit beta
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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