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Mouse Heat Shock Protein Beta 6 (HSPb6) ELISA Kit

SKU:
MODL00644
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q5EBG6
Sensitivity:
0.067ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
HSP20,Heat Shock Protein 20 kD,Heat Shock Protein B6, Alpha Crystallin Related,Heat shock 20 kDa-like protein p20
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Heat Shock Protein Beta 6 (HSPb6) ELISA Kit

The Mouse Heat Shock Protein Beta 6 (HSPB6) ELISA Kit is a reliable tool for accurately measuring levels of HSPB6 in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures dependable and reproducible results, making it suitable for various research applications.HSPB6, also known as the heat shock protein, is involved in stress response and cell protection mechanisms.

It plays a critical role in protecting cells from damage and promoting cell survival under stressful conditions. Studies have shown that HSPB6 is associated with various diseases, including neurodegenerative disorders and cardiovascular diseases, making it a valuable biomarker for research and potential therapeutic interventions.

Product Name:Mouse Heat Shock Protein Beta 6 (HSPb6) ELISA Kit
Product Code:MODL00644
Size:96 Assays
Target:HSPb6
Synonyms:HSP20, Heat Shock Protein 20 kD, Heat Shock Protein B6, Alpha Crystallin Related, Heat shock 20 kDa-like protein p20
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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