Mouse Heat Shock 70kDa Protein 8 (HSPA8) ELISA Kit
The Mouse Heat Shock 70kDa Protein 8 (HSPA8) ELISA Kit is specifically designed for the precise detection of HSPA8 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results, making it an ideal choice for a variety of research endeavors. HSPA8, also known as heat shock cognate 71 kDa protein or HSC70, is a critical chaperone protein involved in protein folding and stress response. It plays a crucial role in maintaining cellular homeostasis and protecting cells from various stressors.
Dysregulation of HSPA8 has been implicated in a range of diseases, including neurodegenerative disorders, cancer, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions. Whether you are investigating the molecular mechanisms of protein folding, stress response pathways, or the role of chaperone proteins in disease, the Mouse HSPA8 ELISA Kit provides a powerful tool for accurate and reliable quantification of HSPA8 levels in mouse samples. Order yours today and take your research to the next level.
Product Name:
Mouse Heat Shock 70kDa Protein 8 (HSPA8) ELISA Kit
Product Code:
MODL00642
Size:
96 Assays
Target:
HSPA8
Synonyms:
LAP1, HSC54, HSC70, HSC71, HSP71, HSPA10, NIP71, HSP73, Heat shock cognate 71 kDa protein, Lipopolysaccharide-associated protein 1, LPS-associated protein 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
