Mouse GZMB Recombinant Protein (RPPB1784)
- SKU:
- RPPB1784
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- P04187
- Research Area:
- Enzymes
Description
| Product Name: | Mouse GZMB Recombinant Protein |
| Product Code: | RPPB1784 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | GZMB |
| Synonyms: | Granzyme B, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E, CGL1, CSPB, CTLA1, GRB, GZMB, CCPI, CGL-1, CSP-B. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GZMB solution (0.25mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4). |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | GEIIGGHEVK PHSRPYMALL SIKDQQPEAI CGGFLIREDF VLTAAHCEGS IINVTLGAHNIKEQEKTQQV IPMVKCIPHP DYNPKTFSND IMLLKLKSKA KRTRAVRPLN LPRRNVNVKPGDVCYVAGWG RMAPMGKYSN TLQEVELTVQ KDRECESYFK NRYNKTNQIC AGDPKTKRASFRGDSGGPLV CKKVAAGIVS YGYKDGSPPR AFTKVSSFLS WIKKTMKSSH HHHHH |
| Biological Activity: | Defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C?. Specific activity is > 9,000 pmol/min/ug. |
GZMB or Granzyme-B is a protein that mainly exist in granules of NK cells and T cells (cytotoxic), it is a serine protease. The protein is secreted by of NK cells and T cells together with the protein that creates pores (perforin) in order to lead to apoptosis in the target cell.
GZMB Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 235 amino acids (19-247 aa) and having a molecular mass of 26.3kDa.GZMB is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily. |
| UniProt Protein Details: | Protein type:EC 3.4.21.79; Protease; Apoptosis Cellular Component: cytoplasm Molecular Function:peptidase activity; protein binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity Biological Process: induction of apoptosis by granzyme; apoptosis; cytolysis; protein processing; proteolysis; T cell mediated cytotoxicity |
| UniProt Code: | P04187 |
| NCBI GenInfo Identifier: | 121584 |
| NCBI Gene ID: | 14939 |
| NCBI Accession: | P04187.1 |
| UniProt Related Accession: | P04187 |
| Molecular Weight: | 27,470 Da |
| NCBI Full Name: | Granzyme B(G,H) |
| NCBI Synonym Full Names: | granzyme B |
| NCBI Official Symbol: | Gzmb�� |
| NCBI Official Synonym Symbols: | GZB; CCP1; Ctla1; Ctla-1; AI553453; CCP-1/C11�� |
| NCBI Protein Information: | granzyme B(G,H); granzyme B(G,H); fragmentin-2; cytotoxic cell protease 1 |
| UniProt Protein Name: | Granzyme B(G,H) |
| UniProt Synonym Protein Names: | CTLA-1; Cytotoxic cell protease 1; CCP1; Fragmentin-2 |
| Protein Family: | Granzyme |
| UniProt Gene Name: | Gzmb�� |
| UniProt Entry Name: | GRAB_MOUSE |
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