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Mouse GTPase HRas (Hras1) ELISA Kit (MOEB1124)

SKU:
MOEB1124
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q61411
Range:
0.156-10 ng/ml
ELISA Type:
Sandwich
Reactivity:
Mouse
€649
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Description

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Mouse GTPase HRas (Hras1) ELISA Kit

The Mouse GTPase HRas/Hras1 ELISA Kit is specifically designed for the precise measurement of HRas/Hras1 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it ideal for a variety of research applications.HRas/Hras1 is a critical GTPase protein involved in signaling pathways regulating cell growth, differentiation, and survival. Dysregulation of HRas/Hras1 has been implicated in various diseases, including cancer and developmental disorders, making it a valuable biomarker for studying these conditions and potentially identifying therapeutic targets.

Overall, the Mouse GTPase HRas/Hras1 ELISA Kit offers researchers a reliable tool for studying the role of HRas/Hras1 in biological processes and diseases, paving the way for new insights and potential treatment strategies.

Product Name:Mouse GTPase HRas (Hras1) ELISA Kit
SKU:MOEB1124
Size:96T
Target:Mouse GTPase HRas (Hras1)
Synonyms:H-Ras-1, Transforming protein p21, c-H-ras, p21ras, Hras1
Assay Type:Sandwich
Detection Method:ELISA
Reactivity:Mouse
Detection Range:0.156-10ng/ml
Sensitivity:0.081ng/mL
Intra CV:Provided with the Kit
Inter CV:Provided with the Kit
Linearity:Provided with the Kit
Recovery:Provided with the Kit
Function:Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Uniprot:Q61411
Sample Type:Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Specificity:Natural and recombinant mouse GTPase HRas
Sub Unit:Forms a signaling complex with RASGRP1 and DGKZ (By similarity). In its GTP-bound form interacts with PLCE1 (By similarity). Interacts with TBC1D10C and RASSF5 (By similarity). Interacts with PDE6D (By similarity). Interacts with IKZF3 (By similarity). Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14 (By similarity). Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain) (By similarity). Interacts with RACK1 (By similarity). Interacts with RAPGEF2 (By similarity). Interacts with RGL3 (PubMed:10869344). Interacts with HSPD1 (PubMed:1347942). Interacts with PIK3CG; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6 (PubMed:19906996). Interacts (in GTP-bound form) with Oog1 (PubMed:16580637).
Research Area:Cancer
Subcellular Location:Cell membrane Cell membrane Lipid-anchor Cytoplasmic side Golgi apparatus Golgi apparatus membrane Lipid-anchor Shuttles between the plasma membrane and the Golgi apparatus. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity).
Storage:Please see kit components below for exact storage details
Note:For research use only
UniProt Protein Function:HRas: a small GTPase protein of the Ras family. Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Mutations are implicated in a variety of human tumors. Protein type: G protein, monomeric; G protein; G protein, monomeric, Ras; Oncoprotein; Motility/polarity/chemotaxisCellular Component: Golgi apparatus; intracellular membrane-bound organelle; plasma membraneMolecular Function: GTP binding; GTPase activity; protein binding; protein C-terminus bindingBiological Process: apoptosis; cell aging; cell cycle arrest; cell proliferation; defense response to protozoan; endocytosis; mitotic cell cycle checkpoint; negative regulation of cell proliferation; negative regulation of neuron apoptosis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of GTPase activity; positive regulation of interferon-gamma production; positive regulation of JNK cascade; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of protein amino acid phosphorylation; positive regulation of Ras protein signal transduction; positive regulation of transcription from RNA polymerase II promoter; protein heterooligomerization; Ras protein signal transduction; regulation of long-term neuronal synaptic plasticity; small GTPase mediated signal transduction; T cell receptor signaling pathway; T-helper 1 type immune response
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q61411
NCBI GenInfo Identifier:341941795
NCBI Gene ID:15461
NCBI Accession:Q61411.2
UniProt Secondary Accession:Q61411,Q6P716, Q80WD2, Q811B9, F7BIB2
UniProt Related Accession:Q61411
Molecular Weight:20,880 Da
NCBI Full Name:GTPase HRas
NCBI Synonym Full Names:Harvey rat sarcoma virus oncogene
NCBI Official Symbol:Hras
NCBI Official Synonym Symbols:ras; H-ras; Hras1; Kras2; Ha-ras; Hras-1; c-H-ras; c-rasHa; c-Ha-ras; Harvey-ras
NCBI Protein Information:GTPase HRas
UniProt Protein Name:GTPase HRas
UniProt Synonym Protein Names:H-Ras-1; Transforming protein p21; c-H-ras; p21ras
Protein Family:GTPase
UniProt Gene Name:Hras
UniProt Entry Name:RASH_MOUSE
Component Quantity (96 Assays) Storage
ELISA Microplate (Dismountable) 8×12 strips -20°C
Lyophilized Standard 2 -20°C
Sample Diluent 20ml -20°C
Assay Diluent A 10mL -20°C
Assay Diluent B 10mL -20°C
Detection Reagent A 120µL -20°C
Detection Reagent B 120µL -20°C
Wash Buffer 30mL 4°C
Substrate 10mL 4°C
Stop Solution 10mL 4°C
Plate Sealer 5 -

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir

*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.

Step
1. Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C.
2. Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform.
3. Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper.
4. Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C.
5. Repeat the wash process for five times as conducted in step 3.
6. Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction.
7. Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
8. Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters.
9. After experiment, store all reagents according to the specified storage temperature respectively until their expiry.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal Fluid Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatant Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysates Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenates The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysates Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast Milk Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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