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Mouse Growth Regulated Oncogene Gamma (GROg) ELISA Kit

SKU:
MODL00587
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q6W5C0
Sensitivity:
25pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
CXCL3,SCYB3,GRO3,GRO-G,MIP2-B,MIP2b,CINC2b,CINC2b,Chemokine(C-X-C-Motif)ligand 3,Macrophage inflammatory protein 2-beta
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Growth Regulated Oncogene Gamma (GROg) ELISA Kit

The Mouse Growth Regulated Oncogene-Gamma (GROG) ELISA Kit is a reliable and sensitive assay designed for the accurate detection of GROG levels in mouse serum, plasma, and cell culture supernatants. This kit provides high specificity and reproducibility, delivering dependable results for a variety of research applications.GROG, also known as CXCL1, is a key chemokine involved in regulating immune responses and inflammation.

It plays a critical role in recruiting immune cells to sites of injury or infection, and dysregulation of GROG levels has been implicated in various disease states, including cancer, inflammation, and autoimmune conditions. The Mouse GROG ELISA Kit enables researchers to study the role of GROG in these conditions and explore potential therapeutic strategies.

Product Name:Mouse Growth Regulated Oncogene Gamma (GROg) ELISA Kit
Product Code:MODL00587
Size:96 Assays
Target:GROg
Synonyms:CXCL3, SCYB3, GRO3, GRO-G, MIP2-B, MIP2b, CINC2b, CINC2b, Chemokine(C-X-C-Motif)ligand 3, Macrophage inflammatory protein 2-beta
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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