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Mouse Growth Differentiation Factor 15 (GDF15) ELISA Kit

SKU:
MODL00538
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z0J7
Sensitivity:
5.8pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
PDF,MIC1,NAG-1,PLAB,PTGFB,Macrophage inhibitory cytokine 1,NSAID-activated gene 1 protein,Placental bone morphogenetic protein,Prostate differentiation factor
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Growth Differentiation Factor 15 (GDF15) ELISA Kit

The Mouse Growth Differentiation Factor 15 (GDF15) ELISA Kit is specifically designed for the accurate detection of GDF15 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and consistent results for various research applications.GDF15 is a vital protein that regulates body weight, metabolism, and inflammation. It plays a key role in various physiological processes, including cell growth, differentiation, and apoptosis.

Elevated levels of GDF15 have been associated with conditions such as obesity, cancer, and cardiovascular diseases, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.Overall, the Mouse GDF15 ELISA Kit offers researchers a reliable tool for investigating the role of GDF15 in physiological and pathological processes in mouse models.

Product Name:Mouse Growth Differentiation Factor 15 (GDF15) ELISA Kit
Product Code:MODL00538
Size:96 Assays
Target:GDF15
Synonyms:PDF, MIC1, NAG-1, PLAB, PTGFB, Macrophage inhibitory cytokine 1, NSAID-activated gene 1 protein, Placental bone morphogenetic protein, Prostate differentiation factor
Detection Method:Sandwich
Range:15.6-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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