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Mouse Granzyme K (GZMK) ELISA Kit

SKU:
MODL00600
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O35205
Sensitivity:
6.5pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
GZM-K,TRYP2,PRSS,Serine Protease,Granzyme 3,Tryptase II,Fragmentin-3,Granzyme-3,NK-tryptase-2
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Granzyme K (GZMK) ELISA Kit

The Mouse Granzyme K (GZMK) ELISA Kit is a highly sensitive and specific assay designed to accurately measure levels of granzyme K in mouse serum, plasma, and cell culture supernatants. Granzyme K is a key protein involved in immune responses, specifically in cytotoxic T cell-mediated killing of target cells.This ELISA kit provides researchers with a reliable and reproducible tool for studying the role of granzyme K in immune function, inflammation, and autoimmune diseases. With its high sensitivity and specificity, this kit is ideal for a wide range of research applications in immunology and oncology.

Granzyme K plays a crucial role in regulating immune responses and has been implicated in various disease states, making it an important biomarker for understanding and potentially targeting immune-related disorders. This ELISA kit is a valuable resource for researchers looking to explore the functions of granzyme K and its potential therapeutic implications.

Product Name:Mouse Granzyme K (GZMK) ELISA Kit
Product Code:MODL00600
Size:96 Assays
Target:GZMK
Synonyms:GZM-K, TRYP2, PRSS, Serine Protease,Granzyme 3, Tryptase II, Fragmentin-3, Granzyme-3, NK-tryptase-2
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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