null

Mouse GPR120  (G Protein Coupled Receptor 120) ELISA Kit  (MOES01806)

SKU:
MOES01806
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q7TMA4
Sensitivity:
0.47ng/mL
Range:
0.78-50ng/mL
ELISA Type:
Sandwich
Reactivity:
Mouse
Sample Type:
Serum, plasma and other biological fluids
Research Area:
Developmental Biology
€649
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

Mouse GPR120 (G Protein Coupled Receptor 120) ELISA Kit

The Mouse GPR120 (G Protein-Coupled Receptor 120) ELISA Kit is specially designed for the accurate quantification of GPR120 levels in mouse serum, plasma, and tissue lysates. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it a valuable tool for various research applications.GPR120 is a key receptor involved in lipid metabolism and inflammatory responses, playing a crucial role in regulating glucose homeostasis and insulin sensitivity.

Dysregulation of GPR120 has been linked to metabolic disorders such as obesity and type 2 diabetes, making it a significant target for therapeutic interventions and drug development.Don't miss out on the opportunity to investigate the role of GPR120 in metabolic diseases and explore potential treatment strategies with the Mouse GPR120 ELISA Kit. Order yours today from Assay Genie!

Assay type:Sandwich
Format:96T
Assay time:4.5h
Reactivity:Mouse
Detection Method:Colormetric
Detection Range:0.78-50 ng/mL
Sensitivity:0.47 ng/mL
Sample Volume Required Per Well:100µL
Sample Type:Serum, plasma and other biological fluids
Specificity:This kit recognizes Mouse GPR120  in samples. No significant cross-reactivity or interference between Mouse GPR120  and analogues was observed.

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GPR120 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GPR120  and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GPR120 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GPR120 . The concentration of Mouse GPR120  in samples can be calculated by comparing the OD of the samples to the standard curve.

UniProt Protein Function:GPR120: Receptor for medium and long-chain free fatty acid (FAA). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti- inflammatory effects particularly in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1 dependent effect but independent of G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediate the taste of fatty acids. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Lipid-binding; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Cellular Component: endocytic vesicle; integral to membrane; integral to plasma membrane; membrane; plasma membrane

Molecular Function:fatty acid binding; G-protein coupled receptor activity; lipid binding; protein binding; signal transducer activity; taste receptor activity

Biological Process: fat cell differentiation; G-protein coupled receptor protein signaling pathway; hormone secretion; negative regulation of apoptosis; negative regulation of cytokine secretion; negative regulation of inflammatory response; signal transduction

UniProt Code:Q7TMA4
NCBI GenInfo Identifier:32306532
NCBI Gene ID:107221
NCBI Accession:NP_861413. 1
UniProt Related Accession:Q7TMA4
Molecular Weight:
NCBI Full Name:free fatty acid receptor 4
NCBI Synonym Full Names:free fatty acid receptor 4
NCBI Official Symbol:Ffar4
NCBI Official Synonym Symbols:Ffa4; GT01; Pgr4; Gpr120; Gpr129; O3far1; KPG_013; AI552415
NCBI Protein Information:free fatty acid receptor 4
UniProt Protein Name:Free fatty acid receptor 4
UniProt Synonym Protein Names:G-protein coupled receptor 120; G-protein coupled receptor GT01; Omega-3 fatty acid receptor 1
Protein Family:Free fatty acid receptor
UniProt Gene Name:Ffar4
UniProt Entry Name:FFAR4_MOUSE

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e. g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration
(ng/mL)		O.DAverageCorrected
502.314
2.356		2.3352.248
251.65
1.654		1.6521.565
12.50.915
0.887		0.9010.814
6.250.443
0.471		0.4570.37
3.130.3
0.28		0.290.203
1.570.201
0.173		0.1870.1
0.780.134
0.142		0.1380.051
00.08
0.094		0.087--

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level MouseGPR120 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level MouseGPR120 were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (ng/mL)2.774.8316.952.844.9516.99
Standard deviation0.190.260.900.170.220.78
C V (%)6.865.385.315.994.444.59

Recovery

The recovery of MouseGPR120 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum (n=5)85-9791
EDTA plasma (n=5)90-10297
Cell culture media (n=5)93-109100

Linearity

Samples were spiked with high concentrations of MouseGPR120 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)91-10196-11185-98
Average (%)9610291
1:4Range (%)87-9880-9490-100
Average (%)938795
1:8Range (%)87-10282-9586-99
Average (%)938892
1:16Range (%)92-10681-9286-101
Average (%)998693

An unopened kit can be stored at 4°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
Reference Standard 2 vials
Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 µL
Concentrated HRP Conjugate (100×) 1 vial, 120 µL -20°C(shading light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 4°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 4°C(shading light)
Stop Solution 1 vial, 10 mL 4°C
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy
  1. Set standard, test sample and control (zero) wells on the pre-coated plate and record theirpositions. It is recommended to measure each standard and sample in duplicate. Note: addall solutions to the bottom of the plate wells while avoiding contact with the well walls. Ensuresolutions do not foam when adding to the wells.
  2. Aliquot 100µl of standard solutions into the standard wells.
  3. Add 100µl of Sample / Standard dilution buffer into the control (zero) well.
  4. Add 100µl of properly diluted sample (serum, plasma, tissue homogenates and otherbiological fluids) into test sample wells.
  5. Cover the plate with the sealer provided in the kit and incubate for 90 min at 37°C.
  6. Aspirate the liquid from each well, do not wash. Immediately add 100µL of BiotinylatedDetection Ab working solution to each well. Cover the plate with a plate seal and gently mix. Incubate for 1 hour at 37°C.
  7. Aspirate or decant the solution from the plate and add 350µL of wash buffer to each welland incubate for 1-2 minutes at room temperature. Aspirate the solution from each well andclap the plate on absorbent filter paper to dry. Repeat this process 3 times. Note: a microplatewasher can be used in this step and other wash steps.
  8. Add 100µL of HRP Conjugate working solution to each well. Cover with a plate seal andincubate for 30 min at 37°C.
  9. Aspirate or decant the solution from each well. Repeat the wash process for five times asconducted in step 7.
  10. Add 90µL of Substrate Reagent to each well. Cover with a new plate seal and incubate forapproximately 15 min at 37°C. Protect the plate from light. Note: the reaction time can beshortened or extended according to the actual color change, but not by more than 30min.
  11. Add 50 µL of Stop Solution to each well. Note: Adding the stop solution should be done inthe same order as the substrate solution.
  12. Determine the optical density (OD value) of each well immediately with a microplate readerset at 450 nm.

Related Products