The Mouse gp130 (Glucoprotein 130) ELISA Kit is a specialized assay designed to quantitatively measure the levels of gp130 in various biological samples.
gp130, a crucial signaling receptor within the interleukin-6 (IL-6) family, plays a pivotal role in mediating diverse cellular processes, including immune responses, inflammation, and cell growth regulation. Through its involvement in intracellular signal transduction pathways, gp130 contributes to the modulation of critical biological functions and the maintenance of physiological homeostasis. Accurate quantification of gp130 levels is essential for unraveling its complex role in cellular signaling cascades, immune responses, and disease pathways. Understanding the precise expression and regulation of gp130 can provide valuable insights into the mechanisms underlying inflammatory conditions, autoimmune disorders, and various pathological states where aberrant signaling occurs. The Mouse gp130 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured with rigorous quality control standards, this kit offers robust performance and user-friendly protocols, making it an excellent tool for researchers seeking to explore the functions of gp130 in biological systems.
Product Name:
Mouse gp130 (Glucoprotein 130) ELISA Kit
SKU:
AEES00261
Target:
Mouse gp130 (Glucoprotein 130)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse gp130. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse gp130 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse gp130, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse gp130. You can calculate the concentration of Mouse gp130 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-105
95-110
97-112
Average (%)
100
100
104
1:4
Range (%)
91-106
90-103
94-108
Average (%)
97
96
99
1:8
Range (%)
94-107
91-105
97-110
Average (%)
100
98
104
1:16
Range (%)
94-106
90-106
96-107
Average (%)
100
97
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.87
17.45
97.93
10.81
16.93
96.75
Standard deviation
0.59
0.96
3.53
0.56
1.01
4.35
C V (%)
5.45
5.49
3.6
5.21
5.98
4.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse gp130 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse gp130 in samples. No significant cross-reactivity or interference between Mouse gp130 and analogues was observed.
