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Mouse Gonadotropin Releasing Hormone Receptor (GnRHR) ELISA Kit

SKU:
MODL00565
Product Type:
ELISA Kit
Sensitivity:
0.116ng/mL
Range:
0.39-25ng/mL
ELISA Type:
Sandwich
Synonyms:
GnRH-R,LRHR,Luteinizing Hormone Releasing Hormone Receptor
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Gonadotropin Releasing Hormone Receptor (GnRHR) ELISA Kit

The Mouse Gonadotropin-Releasing Hormone Receptor (GNRHR) ELISA Kit is specifically engineered for precise detection of GNRHR levels in mouse serum, plasma, and cell culture supernatants. This innovative kit offers unmatched sensitivity and specificity, guaranteeing dependable and consistent results for various research purposes.GNRHR is a key receptor in the endocrine system, regulating the release of gonadotropins and ultimately influencing reproductive function. Dysregulation of GNRHR has been implicated in conditions such as infertility, hormonal disorders, and reproductive cancers, making it a pivotal biomarker for exploring these health concerns and potentially discovering therapeutic interventions.

Don't miss out on the opportunity to enhance your research with the Mouse GNRHR ELISA Kit, providing unparalleled accuracy and efficiency in GNRHR detection. Order yours today and uncover new insights into the intricate mechanisms of reproductive health.

Product Name:Mouse Gonadotropin Releasing Hormone Receptor (GnRHR) ELISA Kit
Product Code:MODL00565
Size:96 Assays
Target:GnRHR
Synonyms:GnRH-R, LRHR, Luteinizing Hormone Releasing Hormone Receptor
Detection Method:Sandwich
Range:0.39-25ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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