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Mouse Glutaminase 2 (GLS2) ELISA Kit

SKU:
MODL00560
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q571F8
Sensitivity:
0.25ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
GA,GLS,LGA,hLGA,L-glutaminase,L-glutamine amidohydrolase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Glutaminase 2 (GLS2) ELISA Kit

The Mouse GLS2 (Glutaminase 2) ELISA Kit from Assay Genie is a powerful tool for the quantitative measurement of GLS2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.GLS2 is an enzyme involved in glutamine metabolism, playing a critical role in cellular energy production and maintaining cellular homeostasis. Dysregulation of GLS2 has been linked to various diseases, including cancer, metabolic disorders, and neurodegenerative conditions.

By accurately measuring GLS2 levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.With the Mouse GLS2 ELISA Kit, researchers can confidently study the role of GLS2 in health and disease, paving the way for advances in understanding and treating a wide range of conditions.

Product Name:Mouse Glutaminase 2 (GLS2) ELISA Kit
Product Code:MODL00560
Size:96 Assays
Target:GLS2
Synonyms:GA, GLS, LGA, hLGA, L-glutaminase, L-glutamine amidohydrolase
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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