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Mouse Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) ELISA Kit

SKU:
MODL00584
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q01097
Sensitivity:
0.140ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
NMDAR2B,NR2B,hNR3,Glutamate [NMDA] receptor subunit epsilon-2,N-methyl D-aspartate receptor subtype 2B,N-methyl-D-aspartate receptor subunit 3
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

Mouse Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) ELISA Kit

The Mouse Glutamate Receptor Ionotropic N-methyl-D-aspartate 2B (GRIN2B) ELISA Kit is specifically designed for the accurate quantification of GRIN2B levels in mouse serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.GRIN2B is a key subunit of the NMDA receptor, playing a crucial role in synaptic plasticity and memory formation. Dysregulation of GRIN2B has been implicated in various neurological disorders, including Alzheimer's disease, schizophrenia, and epilepsy.

Therefore, measuring GRIN2B levels can provide valuable insights into the underlying mechanisms of these conditions and aid in the development of potential therapeutic interventions.Overall, the Mouse GRIN2B ELISA Kit is a valuable tool for researchers studying the role of glutamate receptors in synaptic function and neurological diseases. Its reliable performance and accurate measurements make it an essential component of any research project focusing on GRIN2B biology.

Product Name:Mouse Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) ELISA Kit
Product Code:MODL00584
Size:96 Assays
Target:GRIN2B
Synonyms:NMDAR2B, NR2B, hNR3, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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