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Mouse Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A) ELISA Kit

SKU:
MODL00583
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P35436
Sensitivity:
0.045ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
NMDAR2A,NR2A,Glutamate [NMDA] receptor subunit epsilon-1,N-methyl D-aspartate receptor subtype 2A
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A) ELISA Kit

The Mouse Glutamate Receptor Ionotropic N-Methyl-D-Aspartate 2A (GRIN2A) ELISA Kit is a reliable and accurate assay designed for the quantitative measurement of GRIN2A levels in mouse serum, plasma, and cell lysates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.GRIN2A is a key component of the N-methyl-D-aspartate (NMDA) receptor complex, playing a vital role in excitatory neurotransmission and synaptic plasticity in the central nervous system. Dysregulation of GRIN2A has been implicated in various neurological disorders, including epilepsy, schizophrenia, and Alzheimer's disease, making it a valuable target for studying these conditions and developing therapeutic interventions.

By accurately quantifying GRIN2A levels, researchers can gain valuable insights into the molecular mechanisms underlying neurological disorders and identify potential novel therapeutic targets. The Mouse GRIN2A ELISA Kit is an essential tool for advancing research in the field of neuroscience and drug discovery.

Product Name:Mouse Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A) ELISA Kit
Product Code:MODL00583
Size:96 Assays
Target:GRIN2A
Synonyms:NMDAR2A, NR2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A
Detection Method:Sandwich
Range:0.156-10 ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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