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Mouse Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3) ELISA Kit

SKU:
MODL00580
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z2W9
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GLUR-K3,GLUR3,GLURC,gluR-C,AMPA-selective glutamate receptor 3
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3) ELISA Kit

The Mouse Glutamate Receptor Ionotropic AMPA 3 (GRIA3) ELISA Kit is specifically designed for the precise detection of GRIA3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.GRIA3 is a key protein involved in regulating the function of AMPA receptors in the brain, which play a critical role in synaptic transmission and plasticity.

Dysregulation of GRIA3 has been linked to neurological disorders such as epilepsy, Alzheimer's disease, and schizophrenia, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse GRIA3 ELISA Kit provides researchers with a reliable tool to explore the role of GRIA3 in neurological function and disease pathogenesis, leading to advancements in understanding and treatment options for these complex disorders.

Product Name:Mouse Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3) ELISA Kit
Product Code:MODL00580
Size:96 Assays
Target:GRIA3
Synonyms:GLUR-K3, GLUR3, GLURC, gluR-C, AMPA-selective glutamate receptor 3
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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