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Mouse Glutamate Decarboxylase 1, Brain (GAD1) ELISA Kit

SKU:
MODL00515
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P48318
Sensitivity:
0.31ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
GAD67,SCP,67 kDa glutamic acid decarboxylase,Glutamate decarboxylase 67 kDa isoform
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Glutamate Decarboxylase 1, Brain (GAD1) ELISA Kit

The Mouse Glutamate Decarboxylase 1 (GAD1) ELISA Kit is specifically designed for the accurate measurement of GAD1 levels in mouse brain tissue samples. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.GAD1 is an important enzyme involved in the conversion of glutamate to gamma-aminobutyric acid (GABA) in the brain, playing a crucial role in regulating neurotransmission and maintaining the balance of excitatory and inhibitory signals.

Dysregulation of GAD1 has been implicated in various neurological disorders, such as epilepsy, schizophrenia, and Parkinson's disease, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With easy-to-follow protocols and comprehensive technical support, the Mouse GAD1 ELISA Kit is a valuable tool for researchers studying neurobiology and exploring the role of GAD1 in brain function and disease.

Product Name:Mouse Glutamate Decarboxylase 1, Brain (GAD1) ELISA Kit
Product Code:MODL00515
Size:96 Assays
Target:GAD1
Synonyms:GAD67, SCP, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform
Detection Method:Sandwich
Range:0.78-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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