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Mouse Glutamate Cysteine Ligase, Modifier Subunit (GCLM) ELISA Kit

SKU:
MODL00534
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O09172
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GLCLR,GECS,GCL,Gamma Glutamylcysteine Synthetase,Glutamate Cysteine Ligase Regulatory,Gamma-ECS regulatory subunit,Glutamate cysteine ligase modifier subunit
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Glutamate Cysteine Ligase, Modifier Subunit (GCLM) ELISA Kit

The Mouse Glutamate Cysteine Ligase Modifier Subunit (GCLM) ELISA Kit is specifically designed for the accurate measurement of GCLM levels in mouse samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring reliable and reproducible results for your research needs.GCLM is an essential enzyme involved in the synthesis of glutathione, a key antioxidant that plays a crucial role in protecting cells from oxidative stress and maintaining cellular homeostasis.

Dysregulation of GCLM has been implicated in various diseases such as cancer, liver diseases, and neurological disorders, highlighting its significance as a potential biomarker for disease progression and therapeutic intervention.This Mouse GCLM ELISA Kit provides a valuable tool for researchers to investigate the role of GCLM in various biological processes and disease states, ultimately leading to a better understanding of its therapeutic potential.

Product Name:Mouse Glutamate Cysteine Ligase, Modifier Subunit (GCLM) ELISA Kit
Product Code:MODL00534
Size:96 Assays
Target:GCLM
Synonyms:GLCLR, GECS, GCL, Gamma Glutamylcysteine Synthetase, Glutamate Cysteine Ligase Regulatory, Gamma-ECS regulatory subunit, Glutamate cysteine ligase modifier subunit
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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