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Mouse Glucosidase Beta, Acid (GbA) ELISA Kit

SKU:
MODL00530
Product Type:
ELISA Kit
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GBA1,GCB,GLUC,Imiglucerase,Alglucerase,Beta-glucocerebrosidase,Glucosylceramidase,Œ?-Glucosidase,D-Glucosyl-N-Acylsphingosine Glucohydrolase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Glucosidase Beta, Acid (GbA) ELISA Kit

The Mouse Glucosidase Beta Acid (GBA) ELISA Kit is specifically designed for the quantitative detection of GBA levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.GBA is an enzyme that plays a vital role in the breakdown of glucocerebroside. Mutations in the GBA gene are associated with Gaucher's disease, a rare genetic disorder characterized by the accumulation of glucocerebroside in cells, leading to various symptoms such as hepatosplenomegaly, anemia, and bone abnormalities.

The GBA ELISA Kit can help researchers study the role of GBA in disease development and progression, as well as evaluate potential therapeutic interventions.

Product Name:Mouse Glucosidase Beta, Acid (GbA) ELISA Kit
Product Code:MODL00530
Size:96 Assays
Target:GbA
Synonyms:GBA1, GCB, GLUC, Imiglucerase, Alglucerase, Beta-glucocerebrosidase, Glucosylceramidase, ?-Glucosidase, D-Glucosyl-N-Acylsphingosine Glucohydrolase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9889
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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