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Mouse Glucose 6 Phosphate Dehydrogenase (G6PD) ELISA Kit

SKU:
MODL00513
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q00612
Sensitivity:
0.137ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
Glucose-6-phosphate 1-dehydrogenase
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

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Mouse Glucose 6 Phosphate Dehydrogenase (G6PD) ELISA Kit

The Mouse Glucose-6-Phosphate Dehydrogenase (G6PD) ELISA Kit is specifically designed for the accurate and precise measurement of G6PD levels in mouse serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring reliable results for a variety of research applications.G6PD is an essential enzyme in the pentose phosphate pathway, playing a critical role in protecting cells from oxidative stress and maintaining redox balance. Dysregulation of G6PD has been linked to various diseases, including malaria, diabetes, and hemolytic anemia.

Therefore, the measurement of G6PD levels is crucial for understanding these conditions and developing targeted therapies.With a simple and user-friendly protocol, the Mouse G6PD ELISA Kit provides researchers with a valuable tool for studying the role of G6PD in disease pathogenesis and exploring its potential as a therapeutic target. Trust in the reliability and accuracy of this kit for all your G6PD research needs.

Product Name:Mouse Glucose 6 Phosphate Dehydrogenase (G6PD) ELISA Kit
Product Code:MODL00513
Size:96 Assays
Target:G6PD
Synonyms:Glucose-6-phosphate 1-dehydrogenase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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