The Mouse GLP-1 (Glucagon Like Peptide 1) ELISA Kit is a state-of-the-art assay designed for the precise and quantitative detection of GLP-1 levels in various mouse biological samples. GLP-1 plays a crucial role in glucose metabolism, insulin secretion, and satiety, positioning it as a key regulator of blood sugar levels and metabolism. Accurate measurement of GLP-1 is vital for investigating its impact on metabolic processes and exploring its therapeutic potential in metabolic disorders like diabetes.
By utilizing the Mouse GLP-1 ELISA Kit from Assay Genie, researchers can access exceptional sensitivity and specificity, ensuring accurate and reproducible results. This kit is manufactured under stringent quality control standards to deliver robust performance and reliable data for research exploring the intricate mechanisms of GLP-1 in metabolic regulation and related pathologies. Its user-friendly design makes it a valuable tool for in-depth investigations into the role of GLP-1 in metabolic pathways.
Product Name:
Mouse GLP-1 (Glucagon Like Peptide 1) ELISA Kit
SKU:
AEES00207
Target:
Mouse GLP-1 (Glucagon Like Peptide 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GLP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GLP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GLP-1. You can calculate the concentration of Mouse GLP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
85-99
86-99
Average (%)
95
93
92
1:4
Range (%)
92-106
94-109
86-99
Average (%)
100
102
93
1:8
Range (%)
95-108
91-106
84-95
Average (%)
103
98
92
1:16
Range (%)
96-107
95-105
92-105
Average (%)
101
101
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
91
EDTA plasma (n=5)
93-102
96
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.24
4.27
0.43
1.19
4.32
Standard deviation
0.03
0.06
0.2
0.02
0.06
0.21
C V (%)
6
4.84
4.68
4.65
5.04
4.86
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse GLP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GLP-1 in samples. No significant cross-reactivity or interference between Mouse GLP-1 and analogues was observed.
