Mouse GLO1 Recombinant Protein (RPPB1696)
- SKU:
- RPPB1696
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- Q9CPU0
- Research Area:
- Enzymes
Description
| Product Name: | Mouse GLO1 Recombinant Protein |
| Product Code: | RPPB1696 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | GLO1 |
| Synonyms: | Lactoylglutathionelyase, Aldoketomutase, Glyoxalase I, Glx I, Ketone-aldehyde mutase, Methylglyoxalase,S-D-lactoylglutathione methylglyoxal lyase. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | GLO1protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYTRVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTA WTFSRKATLE LTHNWGTEDD ETQSYHNGNSDPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKIATIILEHHHH HH |
| Biological Activity: | Specific activity is > 210 units/mg, and is defined asthe amount of enzyme that will form 1.0 �mol of S-lactoylglutathione frommethylglyoxal and reduced glutathione per minute at pH 6.5 at 25C. |
GLO1 is involved in the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. GLO1 is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. GLO1 enzyme is ubundantly expressed and present in numerous tumor cell lines, in which its concentration is often upregulated ubiquitisly. GLO1 is a major susceptible gene for autism in an ethnic Chinese population from Taiwan. GLO1 might be involved in the pathophysiology of mood disorders. GLO1 plays a role in the pathophysiology of mood disorders. Overexpression of GLO1 is associated with kidney tumor.
GLO1 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 192 amino acids (1-184a.a.) and having a molecular mass of 21.8kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).GLO1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GLO1: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B. Belongs to the glyoxalase I family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - pyruvate; EC 4.4.1.5; Lyase Chromosomal Location of Human Ortholog: 17 A3.3|17 15.69 cM Cellular Component: cytosol; nucleus; plasma membrane Molecular Function:lactoylglutathione lyase activity; lyase activity; metal ion binding; zinc ion binding Biological Process: carbohydrate metabolic process; glutathione metabolic process; methylglyoxal catabolic process to D-lactate; methylglyoxal metabolic process; negative regulation of apoptosis; osteoclast differentiation; regulation of transcription from RNA polymerase II promoter |
| UniProt Code: | Q9CPU0 |
| NCBI GenInfo Identifier: | 165932333 |
| NCBI Gene ID: | 109801 |
| NCBI Accession: | NP_001107032.1 |
| UniProt Secondary Accession: | Q9CPU0,Q543L3, Q8R3T1, |
| UniProt Related Accession: | Q9CPU0 |
| Molecular Weight: | 20,810 Da |
| NCBI Full Name: | lactoylglutathione lyase |
| NCBI Synonym Full Names: | glyoxalase 1 |
| NCBI Official Symbol: | Glo1�� |
| NCBI Official Synonym Symbols: | GLY1; Qglo; Glo-1; Glo-1r; Glo-1s; Glo1-r; Glo1-s; AW550643; 0610009E22Rik; 1110008E19Rik; 2510049H23Rik�� |
| NCBI Protein Information: | lactoylglutathione lyase |
| UniProt Protein Name: | Lactoylglutathione lyase |
| UniProt Synonym Protein Names: | Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase |
| UniProt Gene Name: | Glo1�� |
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