The Mouse GKN2 (Gastrokine 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of Gastrokine 2 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Gastrokine 2 is a key protein that plays a crucial role in gastrointestinal health and is involved in maintaining the integrity of the gastric mucosa.
Dysregulation of Gastrokine 2 has been implicated in gastric diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Mouse GKN2 CLIA Kit offers researchers a powerful tool for investigating the role of Gastrokine 2 in mouse models and advancing our understanding of gastrointestinal health and disease.
Product Name:
Mouse GKN2 (Gastrokine 2) CLIA Kit
SKU:
MOES00286
Target:
Mouse GKN2 (Gastrokine 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GKN2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GKN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GKN2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GKN2. You can calculate the concentration of Mouse GKN2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
95-112
99-111
Average (%)
94
103
104
1:4
Range (%)
98-114
85-98
101-117
Average (%)
104
91
108
1:8
Range (%)
96-110
87-102
100-117
Average (%)
102
93
108
1:16
Range (%)
87-100
98-111
99-113
Average (%)
92
105
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
102
EDTA plasma (n=5)
98-116
106
Cell culture media (n=5)
97-108
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.93
97.0
457.09
54.81
103.01
499.35
Standard deviation
4.82
11.19
45.11
7.11
7.27
44.74
C V (%)
8.77
11.54
9.87
12.97
7.06
8.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GKN2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GKN2 in samples. No significant cross-reactivity or interference between Mouse GKN2 and analogues was observed.
