Mouse GDF7 Recombinant Protein (RPPB0308)
- SKU:
- RPPB0308
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- P43029
- Research Area:
- Cytokines
Description
| Product Name: | Mouse GDF7 Recombinant Protein |
| Product Code: | RPPB0308 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | GDF7 |
| Synonyms: | Growth/differentiation factor 7, GDF-7, Gdf7. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | GDF7 protein was lyophilized from a 0.2�m filtered concentrated solution in 30% Acetonitrile and 0.1% TFA. |
| Solubility: | It is recommended to reconstitute the lyophilized GDF-7 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized GDF7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF-7 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR |
| Biological Activity: | The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5�g/ml, corresponding to a specific activity of > 2000 IU/mg. |
Growth Differentiation Factor-7 (GDF-7) belongs to the BMP family of TGF-b superfamily proteins. GDF7 elicits its bioactivity via a heterodimeric receptor complex comprised of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor. GDF7 signaling results in the phosphorylation and activation of Smad proteins. GDF-7 is also involved in tendon and ligament formation and repair. In addition, GDF7 regulates bone formation, mesenchymal stem cell differentiation, neuronal differentiation, and axon guidance.
GDF7 Mouse Recombinant produced in E.coli is a non-glycosylated disulfide linked homodimer containing 2 chains of 146 amino acids and having a molecular mass of 29.8kDa.The GDF-7 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GDF7: May play an active role in the motor area of the primate neocortex. Belongs to the TGF-beta family. |
| UniProt Protein Details: | Protein type:Cell development/differentiation; Cytokine; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 12|12 A1.1 Cellular Component: extracellular region; extracellular space Molecular Function:cytokine activity; protein binding; protein homodimerization activity; transforming growth factor beta receptor binding Biological Process: activin receptor signaling pathway; axon guidance; BMP signaling pathway; branching morphogenesis of a tube; cell fate commitment; epithelial cell differentiation; forebrain morphogenesis; gland morphogenesis; midbrain development; neural tube development; positive regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of MAPKKK cascade; reproductive structure development; roof plate formation; spinal cord association neuron differentiation |
| NCBI Summary: | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein may play a role in the differentiation of tendon cells and spinal cord interneurons. Mice lacking a functional copy of this gene exhibit absence of some spinal dopaminergic neurons and brain defects, male sterility, and premature death. [provided by RefSeq, Sep 2016] |
| UniProt Code: | P43029 |
| NCBI GenInfo Identifier: | 919184464 |
| NCBI Gene ID: | 238057 |
| NCBI Accession: | NP_001299805.1 |
| UniProt Secondary Accession: | P43029,Q7TNX4, Q99MY1, |
| UniProt Related Accession: | P43029 |
| Molecular Weight: | 47,106 Da |
| NCBI Full Name: | growth/differentiation factor 7 isoform 1 preproprotein |
| NCBI Synonym Full Names: | growth differentiation factor 7 |
| NCBI Official Symbol: | Gdf7�� |
| NCBI Official Synonym Symbols: | BMP12�� |
| NCBI Protein Information: | growth/differentiation factor 7 |
| UniProt Protein Name: | Growth/differentiation factor 7 |
| Protein Family: | Growth/differentiation factor |
| UniProt Gene Name: | Gdf7�� |
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