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Mouse Galectin 7 (GAL7) ELISA Kit

SKU:
MODL00523
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9CRB1
Sensitivity:
0.049ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
LGALS7,PIG1,TP53I1,HKL-14,Lectin Galactoside-Binding Soluble 7,p53-induced gene 1 protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Galectin 7 (GAL7) ELISA Kit

The Mouse Galectin-7 (Gal7) ELISA Kit is specifically designed for the precise quantification of galectin-7 levels in mouse serum, plasma, and tissue lysates. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Galectin-7 is a protein that plays a key role in various biological processes, including cell adhesion, apoptosis, and immune response modulation.

Its dysregulation has been linked to several diseases, making it a valuable biomarker for studying and potentially targeting these conditions.With its advanced technology and reliable performance, the Mouse Galectin-7 (Gal7) ELISA Kit is an indispensable tool for researchers seeking to uncover the role of galectin-7 in health and disease.

Product Name:Mouse Galectin 7 (GAL7) ELISA Kit
Product Code:MODL00523
Size:96 Assays
Target:GAL7
Synonyms:LGALS7, PIG1, TP53I1, HKL-14, Lectin Galactoside-Binding Soluble 7, p53-induced gene 1 protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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