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Mouse Galectin 1 (GAL1) ELISA Kit

SKU:
MODL00517
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P16045
Sensitivity:
0.31ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
LGALS1,GBP,HLBP14,Galaptin,Lectin,Galactoside-Binding Soluble 1,14 kDa laminin-binding protein,Lactose-binding lectin 1,Putative MAPK-activating protein PM12,S-Lac lectin
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Galectin 1 (GAL1) ELISA Kit

The Mouse Galectin-1 (Gal1) ELISA Kit is specially designed to accurately detect levels of Galectin-1 in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.Galectin-1 is a key protein involved in immune regulation, inflammation, and cell adhesion. It plays a crucial role in various physiological and pathological processes, making it a valuable biomarker for studying immune responses, cancer progression, and other diseases.

The Mouse Galectin-1 (Gal1) ELISA Kit provides researchers with a reliable tool for investigating the role of Galectin-1 in different biological pathways and developing potential therapeutic strategies.

Product Name:Mouse Galectin 1 (GAL1) ELISA Kit
Product Code:MODL00517
Size:96 Assays
Target:GAL1
Synonyms:LGALS1, GBP, HLBP14, Galaptin, Lectin,Galactoside-Binding Soluble 1, 14 kDa laminin-binding protein, Lactose-binding lectin 1, Putative MAPK-activating protein PM12, S-Lac lectin
Detection Method:Sandwich
Range:0.78 -50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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