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Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

SKU:
MODL00511
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P39688
Sensitivity:
0.115ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SLK,SYN,c-Fyn,Src-like kinase
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

The Mouse FYN (Oncogene Related to SRC, FGR, YES) ELISA Kit is a valuable tool for researchers studying the FYN oncogene in mouse models. This kit allows for the accurate detection of FYN levels in mouse serum, plasma, and cell culture supernatants with high sensitivity and specificity. The FYN oncogene is a member of the Src family of kinases and plays a crucial role in regulating cell growth, differentiation, and survival.

Dysregulation of FYN has been implicated in various diseases, including cancer and neurological disorders, making it an important target for research and drug development. With its reliable and reproducible results, the Mouse FYN ELISA Kit is ideal for a wide range of applications, providing researchers with valuable insights into the role of the FYN oncogene in disease pathogenesis and potential therapeutic interventions.

Product Name:Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit
Product Code:MODL00511
Size:96 Assays
Target:FYN
Synonyms:SLK, SYN, c-Fyn, Src-like kinase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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