The Mouse FSH (Follicle Stimulating Hormone) ELISA Kit is a powerful tool for researchers looking to accurately measure FSH levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for a variety of research applications.FSH is a key hormone involved in the regulation of the reproductive system, specifically in the development of ovarian follicles and spermatogenesis.
Dysregulation of FSH levels can lead to infertility and other reproductive disorders, making it a critical biomarker for studying such conditions and potential therapeutic interventions.With the Mouse FSH ELISA Kit, researchers can confidently study the role of FSH in reproductive health and explore new avenues for treatment and intervention. Get accurate and precise results with this advanced ELISA kit from Assay Genie.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FSH. You can calculate the concentration of Mouse FSH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
92-105
85-95
Average (%)
97
99
90
1:4
Range (%)
94-108
82-91
97-112
Average (%)
102
87
103
1:8
Range (%)
95-109
86-99
91-107
Average (%)
101
93
98
1:16
Range (%)
94-111
89-99
93-109
Average (%)
101
94
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
89-106
97
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.7
8.9
43.1
5.1
8.4
40.2
Standard deviation
0.3
0.4
2.1
0.3
0.4
1.7
C V (%)
6.38
4.49
4.87
5.88
4.76
4.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FSH in samples. No significant cross-reactivity or interference between Mouse FSH and analogues was observed.
