The Mouse FRS2 (Fibroblast Growth Factor Receptor Substrate 2) CLIA Kit is specifically designed for the quantification of FRS2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.FRS2 is a critical signaling adaptor protein that plays a key role in the regulation of cell growth, differentiation, and survival. It acts as a key mediator in the fibroblast growth factor (FGF) signaling pathway, influencing various cellular processes such as angiogenesis, tissue repair, and development.
The measurement of FRS2 levels can provide valuable insights into the mechanisms underlying these biological processes and contribute to the development of targeted therapeutic strategies.By utilizing the Mouse FRS2 CLIA Kit, researchers can gain a deeper understanding of the role FRS2 plays in various physiological and pathological conditions, including cancer, cardiovascular diseases, and developmental disorders. This kit serves as a valuable tool for investigating the molecular pathways involved in these conditions and identifying potential therapeutic targets for intervention.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FRS2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FRS2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FRS2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FRS2. You can calculate the concentration of Mouse FRS2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
94-109
95-107
Average (%)
101
100
101
1:4
Range (%)
103-119
100-113
88-102
Average (%)
109
106
93
1:8
Range (%)
88-101
91-106
90-101
Average (%)
93
97
95
1:16
Range (%)
99-112
93-107
95-110
Average (%)
105
100
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
92
EDTA plasma (n=5)
92-105
98
Cell culture media (n=5)
100-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.96
20.59
90.36
9.69
21.68
99.2
Standard deviation
1.12
1.46
8.79
0.84
1.57
8.98
C V (%)
11.24
7.09
9.73
8.67
7.24
9.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FRS2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FRS2 in samples. No significant cross-reactivity or interference between Mouse FRS2 and analogues was observed.
