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Mouse Free Fatty Acid Receptor 3 (FFAR3) ELISA Kit

SKU:
MODL00468
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q3UFD7
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
FFA3R,GPR41,G Protein-Coupled Receptor 41
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Free Fatty Acid Receptor 3 (FFAR3) ELISA Kit

The Mouse FFAR3 (Free Fatty Acid Receptor 3) ELISA Kit is a reliable and accurate tool for detecting FFAR3 levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides researchers with dependable and reproducible results for a variety of research applications.FFAR3 is a G protein-coupled receptor that plays a crucial role in mediating the effects of fatty acids in the body. It is involved in various physiological processes, including regulation of metabolism, immune response, and inflammation.

Dysregulation of FFAR3 has been linked to metabolic disorders, inflammatory diseases, and cancer, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse FFAR3 ELISA Kit offers a comprehensive solution for researchers interested in investigating the role of FFAR3 in health and disease, providing accurate and precise measurements to support their research objectives.

Product Name:Mouse Free Fatty Acid Receptor 3 (FFAR3) ELISA Kit
Product Code:MODL00468
Size:96 Assays
Target:FFAR3
Synonyms:FFA3R, GPR41, G Protein-Coupled Receptor 41
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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